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Competitively Inhibited Interaction Of Inflammatory Transcription
Factors And Importins By Cell-Permeable Nuclear Localization Sequence
Attenuates The Severity Of COVID-19
Dongho Kim, Gyunam Kim, Dasom Shin, Seokwon Lee, Sanghyeon Yu, Misuk Baek, Jaewook Lee,
Hyemin Yu, Joohyun Pi, Mingu Kang, Jieun Kim, Sujeong Kim, Eunna Chung and Daewoong Jo
Cellivery R&D Institute, Cellivery Therapeutics, Inc., Seoul 03929, Korea.
BACKGROUND AIM
The worldwide outbreak of coronavirus disease 19 (COVID-19) is caused Intracellular delivery of NLS is supposed to suppress expression of
by infection with the severe acute respiratory syndrome (SARS)-CoV-2 pro-inflammatory cytokines/chemokines by inhibiting transport of
virus which induces the excessive secretion of pro-inflammatory IATFs from cytoplasm into nucleus. The cell-permeable NLS namely
cytokines, known as “cytokine storm”. The uncontrolled production of pro- iCP-NI regulated expression of cytokines and chemokines. Thus,
inflammatory cytokine due to SARS-CoV-2 infection can be suppressed iCP-NI could be developed as a novel anti-inflammatory immune-
by regulation of nuclear translocation of inflammation-associated therapeutic agent for severe inflammatory diseases including
transcription factors (IATFs) such as NF-κB, STAT1/3, AP-1 & NFAT. COVID-19.
METHODS
To prove the therapeutic efficacy of the iCP-NI in COVID-19, acute respiratory distress syndrome (ARDS) mouse models and SARS-CoV-2
infected golden hamster model were adopted. The LPS, LPS/Poly I:C or SARS-CoV-2 was administrated intratracheally into the animals and
indicated dosage of iCP-NI were treated following optimized treatment protocols.
RESULTS

CONCLUSION REFERENCES Contact Information
iCP-NI significantly attenuates the severity of the SARS- Chung et al. (2020) Science Advances, 6: eaba 1193 Dongho Kim
CoV-2 infected syrian hamster model. The reduction of viral Lim et al. (2013) Clinical Cancer Research, 19: 680-690 Cellivery Therapeutics, Inc.
replication is proved with quantitative PCR by decreasing the Lim et al. (2013) Biomaterials, 34: 6261-6271
level of RdRp gene. Furthermore, the inflammatory cytokine Lim et al. (2012) Molecular Therapy, 20: 1540-1549 kimdh@cellivery.com
genes are significantly decreased and the lung structures Jo et al. (2005) Nature Medicine, 11: 892-898 +82-2-3151-8900
from inflammation-mediated damages are protected. S4-1 신진과학자
Jo et al. (2001) Nature Biotechnology, 19: 929-933